Amylase-producing multiple myeloma: a case report

A 80-year-old man was admitted because of acute-onset thrombocytopenia and renal failure . He was diagnosed with Bence Jones ( lambda ) -type multiple myeloma associated with sepsis with methicillin-resistant Staphylococcus aureus. On admission, serum amylase activity was elevated to 1,814 IU/l (98% salivary type; S-amylase ). Several days after admission, he developed bilateral myelomatous pleuritis. The activity of s-amylase in the effusion was 5,495 IU/l. Myeloma cells in the pleural effusion were positive for cytoplasmic amylase with an antibody against human amylase . High S-amylase activity was detected in the supernatant of cultured myeloma cells in the effusion. Furthermore, s-amylase gene expression was detected by RT-PCR. A diagnosis of amylase-producing multiple myeloma was made. The patient died of renal insufficiency complicated by severe DIC. We report a rare case of amylase-producing myeloma confirmed by immunocytochemistry, culture method, and gene expression.

Mori M, Maruoka H, Nagai Y, Fujita H, Togami K, Tabata S, Kurata M, Matsushita A, Nagai K, Tanaka K, Yamashiro A, Takahashi T.
Department of Hematology and Clinical Immunology, Kobe City Medical Center General Hospital,Rinsho Ketsueki. 2007 Nov;48(11):1484-8.

Complex formation in mixtures of lysozyme-stabilized emulsions and human saliva

In this paper, we studied the interaction between human unstimulated saliva and lysozyme -stabilized oil-in-water emulsions (10 wt/wt% oil phase, 10 mM NaCl, pH 6.7), to reveal the driving force for flocculation of these emulsions. Confocal scanning laser microscopy (CSLM) showed formation of complexes between salivary proteins and lysozyme adsorbed at the oil-water interface and lysozyme in solution as well. To assess the electrostatic nature of the interaction in emulsion/saliva mixtures, laser-diffraction and rheological measurements were conducted in function of the ionic strength by adding NaCl to the mixture in the range between 0 and 168 mM. Increasing the ionic strength reduced the ability of saliva to induce emulsion flocculation as shown by the decreased floc size and the effect on the viscosity. Turbidity experiments with varying pH (3-7) and ionic strength also showed decreased complex formation in mixtures between saliva and lysozyme in solution upon NaCl addition up to 200 mM. Decreasing the pH increased the turbidity, in line with the increase of the positive net charge on the lysozyme molecule. We conclude that electrostatic attraction is the main driving force for complex formation between saliva components and lysozyme adsorbed at the oil droplets and in solution.

Human saliva proteome analysis and disease biomarker discovery

Human saliva is an attractive body fluid for disease diagnosis and prognosis because Human saliva testing is simple, safe, low-cost and noninvasive. Comprehensive analysis and identification of the proteomic content in human whole and ductal saliva will not only contribute to the understanding of oral health and disease pathogenesis, but also form a foundation for the discovery of saliva protein biomarkers for human disease detection. In this article, we have summarized the proteomic technologies for comprehensive identification of proteins in human whole and ductal saliva . We have also discussed potential quantitative proteomic approaches to the discovery of saliva protein biomarkers for human oral and systemic diseases. With the fast development of mass spectrometry and proteomic technologies, we are enthusiastic that saliva protein biomarkers will be developed for clinical diagnosis and prognosis of human diseases in the future.

Cited by
Jianghua Wang, Shen Hu, David T Wong. (2007) Salivary biomarkers for the detection of primary Sjögren’s syndrome. Future Rheumatology 2:5, 447

Human Amylase from Saliva not the band Saliva

Did you know that Lee Biosolutions extensive human donor program allows us to process over 500 liters of human saliva per year for our Human salivary amylase production requirements. We are also able to custom purify to meet your requirements or change a buffer if needed.

I have to share this with you in regards to our human saliva collection program. Earlier this year I received a phone call from a clinical researcher working on a grant with the NIH who said that she was so happy to finally find someone who could custom collect human saliva for her clinical research. Believe me when I say that I try very hard to get the word out about our human saliva program and our efforts in collecting saliva donors with cancer or neurodegenerative disorders such as Saliva From Multiple Sclerosis .

She indicated that every time she typed in saliva into a search engine the band "Saliva" would come up and I should do something about that. I didn't have the heart to tell her that there isn't too much I can do and that I am sure that The Band Saliva has a great deal more groupies than I do. In fact they have their own Wikipedia page Saliva . To say the least I'm pretty impressed. I'm actually going to purchase their CD and wish them the utmost success.

In addition to saliva we are constantly adding to our Biological Fluids product line that also inclues Human Urine and other biologicals. If you can secrete it, there's a protein that can be isolated.


PROSTATE SPECIFIC ANTIGEN
Tumor Markers
MYELOPEROXIDASE
CANCER
ALBUMIN
Vaginal Secretion

Burton Lee
President
http://www.leebio.com/